Reaction: 23S rRNA uridine746 = 23S rRNA pseudouridine746
For diagram of mechanism click here.
Other name(s): RluA (ambiguous); 23S RNA PSI746 synthase; 23S rRNA pseudouridine synthase; pseudouridine synthase RluA (ambiguous)
Systematic name: 23S rRNA-uridine746 uracil mutase
Comments: RluA is the sole protein responsible for the in vivo formation of 23S RNA pseudouridine746 . The dual-specificity enzyme also catalyses the formation of uridine32 in tRNA . cf. EC 22.214.171.124 (tRNA pseudouridine32 synthase).
Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number:
1. Hoang, C., Chen, J., Vizthum, C.A., Kandel, J.M., Hamilton, C.S., Mueller, E.G. and Ferre-D'Amare, A.R. Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure. Mol. Cell 24 (2006) 535-545. [PMID: 17188032]
2. Raychaudhuri, S., Niu, L., Conrad, J., Lane, B.G. and Ofengand, J. Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA. J. Biol. Chem. 274 (1999) 18880-18886. [PMID: 10383384]
3. Wrzesinski, J., Nurse, K., Bakin, A., Lane, B.G. and Ofengand, J. A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi746 in 23S RNA is also specific for Ψ32 in tRNA(phe). RNA 1 (1995) 437-448. [PMID: 7493321]