IUBMB Enzyme Nomenclature


Accepted name: formate—tetrahydrofolate ligase

Reaction: ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate

For diagram of reaction click here (another example).

Other name(s): formyltetrahydrofolate synthetase; 10-formyltetrahydrofolate synthetase; tetrahydrofolic formylase; tetrahydrofolate formylase

Systematic name: formate:tetrahydrofolate ligase (ADP-forming)

Comments: In eukaryotes occurs as a trifunctional enzyme also having methylenetetrahydrofolate dehydrogenase (NADP+) (EC and methenyltetrahydrofolate cyclohydrolase (EC activity.

Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9023-66-9


1. Jaenicke, L. and Brode, E. Untersuchungen über Einkohlenstoffkörper. I. Die Tetrahydrofolatformylase aus Taubenleber. Rinigung und Mechanismus. Biochem. Z. 334 (1961) 108-132.

2. Long, C.W., Levitzki, A., Houston, L.L and Koshland, D.E., Jr. Subunit structures and interactions of CTP synthetase. Fed. Proc. 28 (1969) 342 only.

3. Rabinowitz, J.C. and Pricer, W.E. Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme. J. Biol. Chem. 237 (1962) 2898-2902.

4. Whiteley, H.R., Osborn, M.J. and Huennekens, F.M. Purification and properties of the formate-activating enzyme from Micrococcus aerogenes. J. Biol. Chem. 234 (1959) 1538-1543.

[EC created 1961]

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