IUBMB Enzyme Nomenclature


Accepted name: methylenetetrahydrofolate dehydrogenase (NADP+)

Reaction: 5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH + H+

For diagram of reaction click here (another example).

Other name(s): N5,N10-methylenetetrahydrofolate dehydrogenase; 5,10-methylenetetrahydrofolate:NADP oxidoreductase; 5,10-methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate dehydrogenase (NADP)

Systematic name: 5,10-methylenetetrahydrofolate:NADP+ oxidoreductase

Comments: In eukaryotes, occurs as a trifunctional enzyme also having methenyltetrahydrofolate cyclohydrolase (EC and formate—tetrahydrofolate ligase (EC activity. In some prokaryotes occurs as a bifunctional enzyme also having methenyltetrahydrofolate cyclohydrolase activity (EC

Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9029-14-5


1. Hatefi, Y., Osborn, M.J., Kay, L.D. and Huennekens, F.M. Hydroxymethyl tetrahydrofolic dehydrogenase. J. Biol. Chem. 227 (1957) 637-647.

2. Osborn, M.J. and Huennekens, F.M. Participation of anhydroleucovorin in the hydroxymethyl tetrahydrofolic dehydrogenase system. Biochim. Biophys. Acta 26 (1957) 646-647.

3. Ramasastri, B.V. and Blakley, R.L. 5,10-Methylenetetrahydrofolic acid dehydrogenase from Bakers' yeast. I. Partial purification and some properties. J. Biol. Chem. 237 (1962) 1982-1988.

4. Yeh, Y.-C. and Greenberg, D.M. Purification and properties of N5,N10-methylenetetrahydrofolate dehydrogenase of calf thymus. Biochim. Biophys. Acta 105 (1965) 279-291. [PMID: 4379024]

[EC created 1961]

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