Reaction: [protein]-Nπ-phospho-L-histidine + N-acetyl-D-glucosamine[side 1] = [protein]-L-histidine + N-acetyl-D-glucosamine 6-phosphate[side 2]
Other name(s): nagE (gene name); N-acetyl-D-glucosamine PTS permease; EIINag; Enzyme IINag; EIICBANag
Systematic name: protein-Nπ-phospho-L-histidine:N-acetyl-D-glucosamine Nπ-phosphotransferase
Comments: This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate—protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.
Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number:
References:
1. White, R.J. The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli, Biochem. J. 118 (1970) 89-92. [PMID: 4919472]
2. Rogers, M.J., Ohgi, T., Plumbridge, J. and Soll, D. Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase. Gene 62 (1988) 197-207. [PMID: 3284790]
3. Peri, K.G. and Waygood, E.B. Sequence of cloned enzyme IIN-acetylglucosamine of the phosphoenolpyruvate:N-acetylglucosamine phosphotransferase system of Escherichia coli. Biochemistry 27 (1988) 6054-6061. [PMID: 3056518]
4. Plumbridge, J. An alternative route for recycling of N-acetylglucosamine from peptidoglycan involves the N-acetylglucosamine phosphotransferase system in Escherichia coli, J. Bacteriol. 191 (2009) 5641-5647. [PMID: 19617367]